Danger in the reef: Proteome, toxicity, and neutralization of the venom of the olive sea snake, Aipysurus laevis

Andreas H. Laustens, José Maria Gutiérrez, Arne Redsted Rasmussen, Mikal Engmark, Peter Gravlund, Kate L Sanders, Brian Lohse, Bruno Lomonte

Publikation: Bidrag til tidsskriftTidsskriftartikel

Abstrakt

Four specimens of the olive sea snake, Aipysurus laevis, were collected off the coast of Western Australia, and the venom proteome was characterized and quantitatively estimated by RP-HPLC, SDS-PAGE, and MALDI-TOF-TOF analyses. A. laevis venom is remarkably simple and consists of phospholipases A2 (71.2%), three-finger toxins (3FTx; 25.3%), cysteine-rich secretory proteins (CRISP; 2.5%), and traces of a complement control module protein (CCM; 0.2%). Using a Toxicity Score, the most lethal components were determined to be short neurotoxins. Whole venom had an intravenous LD50 of 0.07 mg/kg in mice and showed a high phospholipase A2 activity, but no proteinase activity in vitro. Preclinical assessment of neutralization and ELISA immunoprofiling showed that BioCSL Sea Snake Antivenom was effective in
cross-neutralizing A. laevis venom with an ED50 of 821 mg venom per mL antivenom, with a binding preference towards short neurotoxins, due to the high degree of conservation between short neurotoxins from A. laevis and Enhydrina schistosa venom. Our results point towards the possibility of developing recombinant antibodies or synthetic inhibitors against A. laevis venom due to its simplicity.
© 2015 Elsevier Ltd. All rights
OriginalsprogEngelsk
TidsskriftToxicon
Vol/bind107, Part B
Udgave nummer1
Sider (fra-til)187-196
Antal sider10
ISSN0041-0101
DOI
StatusUdgivet - jul. 2015

Kunstnerisk udviklingsvirksomhed (KUV)

  • Nej

Citationsformater

Laustens, A. H., Gutiérrez, J. M., Redsted Rasmussen, A., Engmark, M., Gravlund, P., Sanders, K. L., Lohse, B., & Lomonte, B. (2015). Danger in the reef: Proteome, toxicity, and neutralization of the venom of the olive sea snake, Aipysurus laevis. Toxicon, 107, Part B(1), 187-196. https://doi.org/doi:10.1016/j.toxicon.2015.07.008