Abstract
The venom proteome of the monocled cobra, Naja kaouthia, from Thailand, was characterized by RPHPLC, SDS-PAGE, and MALDI-TOF-TOF analyses, yielding 38 different proteins that were either identified or assigned to families. Estimation of relative protein abundances revealed that venom is dominated by three-finger toxins (77.5%; including 24.3% cytotoxins and 53.2% neurotoxins) and phospholipases A2
(13.5%). It also contains lower proportions of components belonging to nerve growth factor, ohanin/ vespryn, cysteine-rich secretory protein, C-type lectin/lectin-like, nucleotidase, phosphodiesterase, metalloproteinase, L-amino acid oxidase, cobra venom factor, and cytidyltransferase protein families. Small amounts of three nucleosides were also evidenced: adenosine, guanosine, and inosine. The most
relevant lethal components, categorized by means of a ‘toxicity score’, were a-neurotoxins, followed by cytotoxins/cardiotoxins. IgGs isolated from a person who had repeatedly self-immunized with a variety of snake venoms were immunoprofiled by ELISA against all venom fractions. Stronger responses against larger toxins, but lower against the most critical a-neurotoxins were obtained. As expected, no
neutralization potential against N. kaouthia venom was therefore detected. Combined, our results display a high level of venom complexity, unveil the most relevant toxins to be neutralized, and provide prospects of discovering human IgGs with toxin neutralizing abilities through use of phage display screening.
© 2015 Elsevier Ltd. All rights reserved.
(13.5%). It also contains lower proportions of components belonging to nerve growth factor, ohanin/ vespryn, cysteine-rich secretory protein, C-type lectin/lectin-like, nucleotidase, phosphodiesterase, metalloproteinase, L-amino acid oxidase, cobra venom factor, and cytidyltransferase protein families. Small amounts of three nucleosides were also evidenced: adenosine, guanosine, and inosine. The most
relevant lethal components, categorized by means of a ‘toxicity score’, were a-neurotoxins, followed by cytotoxins/cardiotoxins. IgGs isolated from a person who had repeatedly self-immunized with a variety of snake venoms were immunoprofiled by ELISA against all venom fractions. Stronger responses against larger toxins, but lower against the most critical a-neurotoxins were obtained. As expected, no
neutralization potential against N. kaouthia venom was therefore detected. Combined, our results display a high level of venom complexity, unveil the most relevant toxins to be neutralized, and provide prospects of discovering human IgGs with toxin neutralizing abilities through use of phage display screening.
© 2015 Elsevier Ltd. All rights reserved.
Original language | English |
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Journal | Toxicon |
Volume | 99 |
Pages (from-to) | 23-35 |
Number of pages | 13 |
ISSN | 0041-0101 |
DOIs | |
Publication status | Published - Mar 2015 |
Artistic research
- No