IDENTIFICATION OF ANIMAL ADHESIVES USING DNA AMPLIFICATION

Anne Marie Eriksen, Hans Viborg Kristensen, Peder Bøllingtoft, Knud Bo Botfeldt, Arne Redsted Rasmussen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The aim of this study was to examine whether DNA was degraded in the manufacturing of animal glue. To test this, two different types of sturgeon glue (Acipenser sp.) were manufactured using historic recipes. One glue was boiled for a substantial amount of time and the other was kept under 75°C. DNA samples were collected from both glues in order to test whether the DNA was degraded in the heating process of making the glue. It was also tested whether two different kinds of flex canvas (for paintings), one coarse and one fine weaved would inhibit the PCR reaction. To do this the glue were applied onto the canvas and samples were collected. To examine the sample size needed to get an amplifiable DNA sample, different sizes were collected of the canvas, 1.0cm2; 0,5cm2 and 0,5cm of a single thread. It was possible to get amplifiable DNA in 11 out of 12 samples collected after the manufacturing of the glue and in 18 out of 24 samples collected of the canvas. In four out of the five cases where it was not possible amplify DNA, the sample belongs to the smallest size of the canvas investigated.
As shown in this study it is possible to get DNA out of boiled animal glue and glue applied onto canvas. The application of a DNA techniques provides several new possibilities for further material analysis of (pre)historic artefacts.
OriginalsprogEngelsk
TidsskriftInternational Journal of Conservation Science
Vol/bind5
Udgave nummer3
Sider (fra-til)369-378
Antal sider10
ISSN2067-533X
StatusUdgivet - sep. 2014

Emneord

  • Animal adhesive; glue; Sturgeon glue; Organic binding media; Isinglass; Acipenser sp.; Huso sp.; Cytochrome b; mtDNA

Kunstnerisk udviklingsvirksomhed (KUV)

  • Nej

Citer dette

Eriksen, Anne Marie ; Kristensen, Hans Viborg ; Bøllingtoft, Peder ; Botfeldt, Knud Bo ; Redsted Rasmussen, Arne. / IDENTIFICATION OF ANIMAL ADHESIVES USING DNA AMPLIFICATION. I: International Journal of Conservation Science. 2014 ; Bind 5, Nr. 3. s. 369-378.
@article{7b91800cee28486c8d86209e2a145272,
title = "IDENTIFICATION OF ANIMAL ADHESIVES USING DNA AMPLIFICATION",
abstract = "The aim of this study was to examine whether DNA was degraded in the manufacturing ofanimal glue. To test this, two different types of sturgeon glue (Acipenser sp.) weremanufactured using historic recipes. One glue was boiled for a substantial amount of time andthe other was kept under 75°C. DNA samples were collected from both glues in order to testwhether the DNA was degraded in the heating process of making the glue. It was also testedwhether two different kinds of flex canvas (for paintings), one coarse and one fine weavedwould inhibit the PCR reaction. To do this the glue were applied onto the canvas and sampleswere collected. To examine the sample size needed to get an amplifiable DNA sample, differentsizes were collected of the canvas, 1.0cm2; 0,5cm2 and 0,5cm of a single thread. It was possibleto get amplifiable DNA in 11 out of 12 samples collected after the manufacturing of the glueand in 18 out of 24 samples collected of the canvas. In four out of the five cases where it wasnot possible amplify DNA, the sample belongs to the smallest size of the canvas investigated.As shown in this study it is possible to get DNA out of boiled animal glue and glue applied ontocanvas. The application of a DNA techniques provides several new possibilities for furthermaterial analysis of (pre)historic artefacts.",
keywords = "Animal adhesive; glue; Sturgeon glue; Organic binding media; Isinglass; Acipenser sp.; Huso sp.; Cytochrome b; mtDNA, animal adhesive, mtDNA, glue, Sturgeon glue, organic binding media",
author = "Eriksen, {Anne Marie} and Kristensen, {Hans Viborg} and Peder B{\o}llingtoft and Botfeldt, {Knud Bo} and {Redsted Rasmussen}, Arne",
year = "2014",
month = "9",
language = "English",
volume = "5",
pages = "369--378",
journal = "International Journal of Conservation Science",
issn = "2067-533X",
publisher = "Alexandru Ioan Cuza University of Iasi",
number = "3",

}

IDENTIFICATION OF ANIMAL ADHESIVES USING DNA AMPLIFICATION. / Eriksen, Anne Marie; Kristensen, Hans Viborg; Bøllingtoft, Peder; Botfeldt, Knud Bo; Redsted Rasmussen, Arne.

I: International Journal of Conservation Science, Bind 5, Nr. 3, 09.2014, s. 369-378.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - IDENTIFICATION OF ANIMAL ADHESIVES USING DNA AMPLIFICATION

AU - Eriksen, Anne Marie

AU - Kristensen, Hans Viborg

AU - Bøllingtoft, Peder

AU - Botfeldt, Knud Bo

AU - Redsted Rasmussen, Arne

PY - 2014/9

Y1 - 2014/9

N2 - The aim of this study was to examine whether DNA was degraded in the manufacturing ofanimal glue. To test this, two different types of sturgeon glue (Acipenser sp.) weremanufactured using historic recipes. One glue was boiled for a substantial amount of time andthe other was kept under 75°C. DNA samples were collected from both glues in order to testwhether the DNA was degraded in the heating process of making the glue. It was also testedwhether two different kinds of flex canvas (for paintings), one coarse and one fine weavedwould inhibit the PCR reaction. To do this the glue were applied onto the canvas and sampleswere collected. To examine the sample size needed to get an amplifiable DNA sample, differentsizes were collected of the canvas, 1.0cm2; 0,5cm2 and 0,5cm of a single thread. It was possibleto get amplifiable DNA in 11 out of 12 samples collected after the manufacturing of the glueand in 18 out of 24 samples collected of the canvas. In four out of the five cases where it wasnot possible amplify DNA, the sample belongs to the smallest size of the canvas investigated.As shown in this study it is possible to get DNA out of boiled animal glue and glue applied ontocanvas. The application of a DNA techniques provides several new possibilities for furthermaterial analysis of (pre)historic artefacts.

AB - The aim of this study was to examine whether DNA was degraded in the manufacturing ofanimal glue. To test this, two different types of sturgeon glue (Acipenser sp.) weremanufactured using historic recipes. One glue was boiled for a substantial amount of time andthe other was kept under 75°C. DNA samples were collected from both glues in order to testwhether the DNA was degraded in the heating process of making the glue. It was also testedwhether two different kinds of flex canvas (for paintings), one coarse and one fine weavedwould inhibit the PCR reaction. To do this the glue were applied onto the canvas and sampleswere collected. To examine the sample size needed to get an amplifiable DNA sample, differentsizes were collected of the canvas, 1.0cm2; 0,5cm2 and 0,5cm of a single thread. It was possibleto get amplifiable DNA in 11 out of 12 samples collected after the manufacturing of the glueand in 18 out of 24 samples collected of the canvas. In four out of the five cases where it wasnot possible amplify DNA, the sample belongs to the smallest size of the canvas investigated.As shown in this study it is possible to get DNA out of boiled animal glue and glue applied ontocanvas. The application of a DNA techniques provides several new possibilities for furthermaterial analysis of (pre)historic artefacts.

KW - Animal adhesive; glue; Sturgeon glue; Organic binding media; Isinglass; Acipenser sp.; Huso sp.; Cytochrome b; mtDNA

KW - animal adhesive

KW - mtDNA

KW - glue

KW - Sturgeon glue

KW - organic binding media

M3 - Journal article

VL - 5

SP - 369

EP - 378

JO - International Journal of Conservation Science

JF - International Journal of Conservation Science

SN - 2067-533X

IS - 3

ER -